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異檸檬酸脱氫酶 (NADP+)

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異檸檬酸脱氫酶 (NADP+)
大肠杆菌E. coli)異檸檬酸脱氫酶(NADP+)的晶体结构(PDB 1CW7)[1] 这种酶有三个活性位点,三分子柠檬酸中的其中一个在NADP+结合区域中。
识别码
EC編號 1.1.1.42
CAS号 9028-48-2
数据库
IntEnz IntEnz浏览
BRENDA英语BRENDA BRENDA入口
ExPASy英语ExPASy NiceZyme浏览
KEGG KEGG入口
MetaCyc英语MetaCyc 代谢路径
PRIAM英语PRIAM_enzyme-specific_profiles 概述
PDB RCSB PDB PDBj PDBe PDBsum
基因本体 AmiGO / EGO

異檸檬酸脱氫酶 (NADP+)(英語:isocitrate dehydrogenase (NADP+)EC 1.1.1.42页面存档备份,存于互联网档案馆))是一种以NAD+NADP+受体、作用于供体CH-OH基团上的氧化还原酶。这种催化以下酶促反应

異檸檬酸 + NADP+ 2-酮戊二酸 + CO2 + NADPH

異檸檬酸脱氫酶 (NADP+)需要Mn2+Mg2+以维持活性。真核细胞中的异柠檬酸脱氢酶 (NAD+)以两种形式存在:其中一种与NADP+还存在于细胞质基质中不能变构调节;另一种与NAD+相关联且仅在线粒体中被发现并但可以变构调节。[2] 这两种酶——异柠檬酸脱氢酶 (NADP+)与异柠檬酸脱氢酶 (NAD+)并不相同,前者能以草酰琥珀酸作为底物。某些物种的异柠檬酸脱氢酶 (NADP+)也能利用NAD+,但催化的反应速率较慢。[3]

参考文献

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  1. ^ Cherbavaz DB, Lee ME, Stroud RM, Koshland DE. Active site water molecules revealed in the 2.1 A resolution structure of a site-directed mutant of isocitrate dehydrogenase. J. Mol. Biol. January 2000, 295 (3): 377–85. PMID 10623532. doi:10.1006/jmbi.1999.3195. 
  2. ^ Camacho, M.L., Brown, R.A., Bonete, M.J., Danson, M.J. and Hough, D.W. Isocitrate dehydrogenases from Haloferax volcanii and Sulfolobus solfataricus: enzyme purification, characterisation and N-terminal sequence. 134. 1995: 85–90 [2012-05-08]. PMID 8593959. (原始内容存档于2019-03-23) (英语).  |journal=被忽略 (帮助)
  3. ^ Steen, I.H., Lien, T. and Birkeland, N.-K. Biochemical and phylogenetic characterization of isocitrate dehydrogenase from a hyperthermophilic archaeon, ‘’Archaeoglobus fulgidus’’.. Arch. Microbiol. 1997, 168: 412–420. PMID 9325430 (英语). 
  • Agosin, M.U. and Weinbach, E.C. Partial purification and characterization of the isocitric dehydrogenase from ‘’Trypanosoma cruzi’’.. Biochim. Biophys. Acta. 1956, 21: 117–126. PMID 13363868 (英语). 
  • Moyle, J. and Dixon, M. Purification of the isocitrate enzyme (triphosphopyridine nucleotide-linked isocitrate dehydrogenase-oxalosuccinate carboxylase).. Biochem. J. 1956, 63: 548–552. PMID 13355848 (英语). 
  • Plaut, G.W.E. Isocitrate dehydrogenases. Lardy, H. and Myrbäck, K. (编). The Enzymes 7 2nd. New York: Academic Press. 1963: 105–126 (英语). 
  • Siebert, G., Dubuc, J., Warner, R.C. and Plaut, G.W.E. The preparation of isocitrate dehydrogenase from mammalian heart.. J. Biol. Chem. 1957, 226: 965–975. PMID 13438885 (英语). 
  • Vickery, H.B. A. suggested new nomenclature for the isomers of isocitric acid. 237. 1962: 1739–1741. PMID 13925783 (英语).  |journal=被忽略 (帮助)
  • Koh, H.J., Lee, S.M., Son, B.G., Lee, S.H., Ryoo, Z.Y., Chang, K.T., Park, J.W., Park, D.C., Song, B.J., Veech, R.L., Song, H. and Huh, T.L. Cytosolic NADP+-dependent isocitrate dehydrogenase plays a key role in lipid metabolism.. 2004: 39968–39974. PMID 15254034 (英语).  |journal=被忽略 (帮助)
  • Ceccarelli, C., Grodsky, N.B., Ariyaratne, N., Colman, R.F. and Bahnson, B.J. Crystal structure of porcine mitochondrial NADP+-dependent isocitrate dehydrogenase complexed with Mn2+ and isocitrate. Insights into the enzyme mechanism.. J. Biol. Chem. 277. 2002: 43454–43462. PMID 12207025 (英语).