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CHO-K1

维基百科,自由的百科全书

CHO-K1CHO cell clone K1)是源自成年中国仓鼠卵巢的二倍体上皮细胞系,并且是CHO细胞(源自中国仓鼠卵巢深入活体组织切片)衍生的野生型亚克隆。目前已知利用甲磺酸乙酯诱变CHO-K1细胞后,会产生缺乏二氢叶酸还原酶(DHFR)活性的CHO-DXB11细胞[1] 。此外,源自CHO-K1细胞的细胞系的基因组可能曾有着大规模的染色体重新排列,甚至已知它的克隆群体也可以分化为异质亚族群(heterogeneous subpopulations)[2][3]

据报导,CHO-K1细胞的染色体计数各有不同[4],可能是因为它的基因组不稳定。有研究就在CHO-K1细胞中发现了21条染色体[5],并且在在CHO-K1细胞的基因组中,鉴定出与大多数人类硫酸乙酰肝素葡糖胺O-磺基转移酶(human heparan sulfate glucosamine O-sulfotransferases)的同源物[5],鉴定结果与以前的研究[6][7]一致。

参考资料

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  1. ^ Urlaub, G; Chasin, LA. Isolation of Chinese hamster cell mutants deficient in dihydrofolate reductase activity.. Proceedings of the National Academy of Sciences of the United States of America. 1980-07, 77 (7): 4216–20 [2020-01-09]. PMID 6933469. doi:10.1073/pnas.77.7.4216. (原始内容存档于2020-02-23). 
  2. ^ M. Derouazi; D. Martinet; N. Besuchet; R. Flaction; M. Wicht; M. Bertschinger; D. Hacker; J. Beckmann; F.M. Wurm. Stability and Cytogenetic Characterization of Recombinant CHO Cell Lines Established by Microinjection and Calcium Phosphate Transfection. Cell Technology for Cell Products. 2007: 443–446. doi:10.1007/978-1-4020-5476-1_73. 
  3. ^ Pilbrough, W; Munro, TP; Gray, P. Intraclonal protein expression heterogeneity in recombinant CHO cells.. PloS one. 2009-12-23, 4 (12): e8432 [2020-02-23]. PMID 20037651. doi:10.1371/journal.pone.0008432. (原始内容存档于2020-02-23). 
  4. ^ Deaven, LL; Petersen, DF. The chromosomes of CHO, an aneuploid Chinese hamster cell line: G-band, C-band, and autoradiographic analyses.. Chromosoma. 1973, 41 (2): 129–44 [2020-02-23]. PMID 4120885. doi:10.1007/bf00319690. (原始内容存档于2020-02-23). 
  5. ^ 5.0 5.1 Xu, X; Nagarajan, H; Lewis, NE; Pan, S; Cai, Z; Liu, X; Chen, W; Xie, M; Wang, W; Hammond, S; Andersen, MR; Neff, N; Passarelli, B; Koh, W; Fan, HC; Wang, J; Gui, Y; Lee, KH; Betenbaugh, MJ; Quake, SR; Famili, I; Palsson, BO; Wang, J. The genomic sequence of the Chinese hamster ovary (CHO)-K1 cell line.. Nature biotechnology. 2011-07-31, 29 (8): 735–41 [2020-02-23]. PMID 21804562. doi:10.1038/nbt.1932. (原始内容存档于2020-02-23). 
  6. ^ Kobayashi, M; Habuchi, H; Yoneda, M; Habuchi, O; Kimata, K. Molecular cloning and expression of Chinese hamster ovary cell heparan-sulfate 2-sulfotransferase.. The Journal of biological chemistry. 1997-05-23, 272 (21): 13980–5 [2020-02-23]. PMID 9153262. doi:10.1074/jbc.272.21.13980. (原始内容存档于2020-02-23). 
  7. ^ Habuchi, H; Kobayashi, M; Kimata, K. Molecular characterization and expression of heparan-sulfate 6-sulfotransferase. Complete cDNA cloning in human and partial cloning in Chinese hamster ovary cells.. The Journal of biological chemistry. 1998-04-10, 273 (15): 9208–13 [2020-02-23]. PMID 9535912. doi:10.1074/jbc.273.15.9208. (原始内容存档于2020-02-23). 

外部链接

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